INTRODUCTION
A simple mixture of 4 compounds was used to evaluate the capacity of the Gilson PLC 2050 to scale up a separation from analytical to semi-preparative HPLC. The analytical method was first developed on a LaChrom Elite™ analytical HPLC system and then transferred to the Gilson PLC 2050(Figure 1). The method is then scaled up 50-fold using the PLC 2050 system to allow for higher throughput separations on a semipreparative HPLC column.(25mm I.D.)
Figure 1. Gilson PLC 2O50 System from Gilson.
MATERIALS & METHODS
LaChrom Elite(Hitachi) is equipped with a 10mL/min quaternary gradient pumping system, Photodiode Array Detector(200-800nm) and EZChrom Elite™ Chromatography Data Software.
PLC 2050(Gilson) is equipped with a 50ml/min quaternary gradient pumping system, UV/Vis detector(200-800nm) and Gilson Glider Prep Software.
Analytical Method is the same for all tests(see Table 1).
Table 1. Experimental conditions used to generate the analytical and preparative chromatograms.
RESULTS & DISCUSSION
In this test, the profile of analytical separation(20uL injection) on the PLC 2050(Figure 2B) was similar to the chromatogram obtained using the LaChrom EliteTM HPLC system(Figure 2A).
Furthermore, the use of a preparative column(1ml injection) with the PLC 2050(Figure 2C) demonstrates clear separation of the compounds.
Figure 2. HPLC Chromatogram at 280nm.
Hydroquinone, pyrocatechol, phenol, and piperine were separated using
A) An analytical column with a LaChrom EliteTM(Hitachi);
B) A an analytical column with a Gilson PLC 2050 personal chromatography system;
C) A preparative column with a Gilson PLC 2050.
SUMMARY
• The Gilson PLC 2050 system allows for reproducible analytical injections on 4.6mm I.D. columns with high efficiency.
• This application note also demonstrates scale up to a 25mm I.D. column.
• It is also possible to use the Gilson PLC products to perform similar scale-up with preparative columns, up to 100mm I.D. (Results not shown).
'Analytical and Semi-preparative Chromatography of Aromatic Compounds on a Single HPLC System'에 대한 궁금한 내용은 본 원고자료를 제공한 한국분석기기(주)를 통하여 확인할 수 있다.
Reference(참고문헌): Application Note ENV 0214 (pp1-2)
Model Name(모델명): PLC 2050 System
The Person in Charge(담당자): Hyesook An
Maker(제조사): Gilson.
Country of Origin(원산지): France
Mail inquiry: kaisco1@kaisco.co.kr
Data Services(자료제공): KAISCO.
이전기사 :Downstream Plasma Technology for Cleaning TEM Samples on Carbon Films
다음기사 :APPLICATION NOTE PlasmaQuant® PQ 9000 Elite